Cross Reactivity between Dromedary Whey Proteins and IgG Anti Bovine α-Lactalbumin and Anti Bovine β-Lactoglobulin
N. Youcef, D. Saidi, F. Mezemaze, K. E. El-Mecherfi, H. Kaddouri, H. Negaoui, A. Chekroun and O. Kheroua
DOI : 10.3844/ajassp.2009.1448.1452
American Journal of Applied Sciences
Volume 6, Issue 8
Problem statement: Our aim was to enhance the data on antigenic properties of dromedary whey proteins. Approach: The identification of the whey proteins was carried by SDS-page and Reversed phase high performance liquid chromatography (RP-HPLC). The cross-reactivity of dromedary whey proteins with IgG anti bovine β-lactoglobulin and anti bovine α-lactalbumin, obtained by immunisation of Balb/c mice, was carried out by ELISA. Results: The SDS-page showed the presence of band corresponding to α-lactalbumin and albumin serum; this was confirmed by the chromatogram obtained by RP-HPLC, where we detected a pick corresponding to α-lactalbumin. There was a cross reaction of dromedary whey proteins with IgG anti bovin α-~
Cite this Article
Youcef, N., D. Saidi, F. Mezemaze, K.E. El-Mecherfi and H. Kaddouri et al., 2009. Cross Reactivity between Dromedary Whey Proteins and IgG Anti Bovine α-Lactalbumin and Anti Bovine β-Lactoglobulin. Am. J. Applied Sci., 6: 1448-1452.