TY  - JOUR
AU  - Martinez-Romero, Aurora 
AU  - Ortega-Sanchez, Jose Luis 
AU  - Hernandez-Salgado, Jose Ramon 
AU  - Mendoza-Ramos, Margarita Yolanda 
AU  - Rodriguez-Martinez, Rafael 
AU  - Cervantes-Flores, Maribel 
AU  - Lopez-Perez, Joel 
AU  - Urtiz-Estrada, Norma 
AU  - Arenas-Vargas, Miguel 
AU  - Villegas-Viscaino, Raul 
PY  - 2009
TI  - Reactive Nitrogen Intermediates Production by Macrophages of Mycobacterium bovis-Infected Goats and Supplemented with Dyhidroxyvitamin D3 in vivo
JF  - American Journal of Animal and Veterinary Sciences
VL  - 4
IS  - 1
DO  - 10.3844/ajavsp.2009.6.13
UR  - https://thescipub.com/abstract/ajavsp.2009.6.13
AB  - Problem statement: Tuberculosis (TB) remains one of the world's major health problems. To evaluate in vivo indices of cellular sensitization, antigen-induced Reactive Nitrogen Intermediates (RNI) responses by blood mononuclear cells from Mycobacterium bovis BCG-infected goats supplemented with 1, 25 dyhidroxyvitamin D3 [1, 25-(OH)2D3]. Approach: An experimental, longitudinal and comparative study was planned. Twelve animals of goat cattle 20-to 24-month-old sannen selected themselves. Five samplings were made, previous to the inoculation (zero d), 3, 7, 14 and 21 d after applying the treatments. The mononuclear cells by the Ficoll-hypaque method were obtained. The RNI, nitrites and nitrates (NO2- and NO3-) were quantified by Enzyme-Linked Immunosorbent Assay (ELISA). Results: The treatment with the 1, 25(OH)2D3 stimulated the NO3- synthesis indicating, that by itself it is a good modulador of the micobacterial replication and in the treatment with M. bovis-BCG vaccine increased as a result to the treatment with 1,25(OH)2D3. The exhibition to M. bovis-BCG vaccine with the treatment with 1, 25(OH)2D3 was able to increase answer NO3- in exposed animals. Conclusion: The 1, 25(OH)2D3 stimulated in vivo the production of RNI in goats exposed to M. bovis BCG vaccine.