TY  - JOUR
AU  - Kaveh, Kamran 
AU  - Ibrahim, Rashid 
AU  - AbuBakar, Mohd Zuki 
AU  - Ibrahim, Tengku Azmi 
PY  - 2010
TI  - Osteoporosis Induction in Animal Model
JF  - American Journal of Animal and Veterinary Sciences
VL  - 5
IS  - 2
DO  - 10.3844/ajavsp.2010.139.145
UR  - https://thescipub.com/abstract/ajavsp.2010.139.145
AB  - Problem statement: Osteoporosis; the major health problem is an imperative disease requires a  significant attention to discover different aspects of this threatening  abnormality. There are some problems in osteoporotic human population to  conduct researches such as lack of a homogenous sample size or ethical  problems. So, there was a need to have protocol in animal model to let the  scientists discover various feature of this disease to promote health status of  the human population. The aim of this research was to make such protocol in  animal model. Approach: About 15  rabbits were utilized in the current research. Six animals of Group 1 have  undergone bilateral ovariectomy which was followed by IM injection of 1 mg kg-1 day of methylprednisolone. Six  rabbits of second group only undergone bilateral ovariectomy and three animal  of Group 3 served as a control group. They neither undergone any surgery nor  received any medications. After surgery up to four weeks radiographs were  taken. At week four animals were euthanized and samples of cortical and chancellors'  bone were evaluated by light and scanning electron microscopes. Results: Group 1 completely manifested  features of osteoporotic bone. Complete radiolucent view with cortical thinning  in radiographs as well as broken, thinned and perforated chancellors bone  trabecullae in light and electron microscope confirmed the osteoporotic nature  of both cortical and chancellors' bone. Ovariectomy alone did not show  sufficient potential to induce osteoporosis in rabbits in such short period. Conclusion: As the conclusion,  bilateral ovariectomy following injection of mild doses of glucocorticoids  could result in a fast, easy and trustable protocol to induce osteoporosis in  rabbit model. This procedure could be utilized in creating osteoporotic rabbits  for better understanding of unknown aspects of the recent growing syndrome.