TY  - JOUR
AU  - Salehi, Mahmood 
AU  - Ghanbarpour, Reza 
PY  - 2010
TI  - Characterization of Escherichia coli Isolates from Commercial Layer Hens with Salpingitis
JF  - American Journal of Animal and Veterinary Sciences
VL  - 5
IS  - 3
DO  - 10.3844/ajavsp.2010.208.214
UR  - https://thescipub.com/abstract/ajavsp.2010.208.214
AB  - Problem statement: Escherichia coli isolates are the most common bacterial agents of
salpingitis in commercial layer hens. The objectives of this study were to determine the phylogenetic
groups/subgroups and antibiotic resistance of E. coli isolates from salpingitis cases in commercial layer
hens farms in southeast of Iran. Approach: One hundred twenty one E. coli isolates from oviducts of
layer hens with salpingitis were examined to determine their O-serogroup, phylogenetic
group/subgroup and antibiotic resistance pattern. Results: O-serogroup determination test showed that
47 (38.84%) isolates were typeable and belonged to eleven different O serogroups including: O1, O2,
O6, O8, O15, O20, O25, O36, O78, O86 and O111 and 71 (58.67%) isolates were O-nontypeable.
Three most prevalent serogroup were O78, O2 and O1 respectively. PCR assays showed that the
isolates fall into four phylogenetic groups A (41.32%), D (33.88%), B2 (14.87%) and B1 (9.91%).
Ninety nine (81.81%) isolates fell into six phylogenetic subgroups including: A0 (17.35%), A1
(23.96%), B22 (5.78%), B23 (9.09%), D1 (13.22%) and D2 (20.66%). All of the E. coli isolates from
O78 serogroup belonged to A phylo-group whereas the O2 isolates mostly fell into D group. The
maximum antibiotic resistance was against tetracycline (100%) and minimum resistance was against
linco-spectin (37.19%). Twenty four isolates (19.83%) were resistant to all of the examined antibiotics.
Twenty three different patterns of multiple drug resistance were observed, out of which N-Te-Sxt-Fm-
Nfx-C and N-Te-Sxt-Gm-Fm-Nfx-C-Ls was the two most frequent patterns respectively. The resistant
isolates were found in all of the phylogenetic groups and or sub groups. Conclusion: There are
similarities between salpingitis derived E. coli strains and other avian pathogenic E. coli isolates in
phylogroups, O-serogroups and antibio-resistance patterns.