TY  - JOUR
AU  - Yan, Yu 
AU  - Yanlin, Feng 
AU  - Jinxin, Feng 
AU  - Kejun, Li 
AU  - Gaojie, Liu 
AU  - Xiangliang, Zhang 
PY  - 2020
TI  - Clinical Significance of Precise Hyperthermic Intraperitoneal Chemotherapy Up-regulation RP11-356I2.2 Restraining the Occurrence and Development of Gastric Cancer
JF  - American Journal of Biochemistry and Biotechnology
VL  - 16
IS  - 2
DO  - 10.3844/ajbbsp.2020.252.259
UR  - https://thescipub.com/abstract/ajbbsp.2020.252.259
AB  - In order to study the therapeutic effect and possible mechanism of Precise Hyperthermic Intraperitoneal Chemotherapy (HIPEC) on gastric cancer, the expression of RP11-356I2.2 in gastric cancer cell lines and tissues was detected by real-time fluorescence quantitative PCR (RT-PCR). Chi-square test was used to analyze the correlation between the expression and clinic pathological parameters. Western blotting was used to detect the expression of RP11-356I2.2 and tumor suppressor geneKLF17 after precise HIPEC. The effect of RP11-356I2.2 on KLF17 protein was observed and expressed by Western blotting and immunofluorescence. COX regression model was used to analyze the relationship between the expression of RP11-356I2.2 and KLF17 and the prognosis of patients with gastric cancer. The results of RT-PCR showed that RP11-356I2.2 was lower in BGC-823, MKN-28, MGC-803, SGC-7901 and MKN-45 than normal stomach cells GES-1 (P