Research Article Open Access

The Effect of Physical Refining on Palm Vitamin E (Tocopherol, Tocotrienol and Tocomonoenol)

Chiew Wei Puah, Yuen May Choo, Ah Ngan Ma and Cheng Hock Chuah

Abstract

This study reports the effect of physical refining on palm vitamin E including α- tocopherol, α-, γ and α-tocotrienols as well as α- tocomonoenol. A method using HPLC with fluorescence detector using normal phase silica column is described. An isocratic elution with n-hexane/THF/2-propanol (1000:60:4, by vol.) as mobile phase was used. The structure of the α- tocomonoenol was determined using gas chromatography coupled with mass spectrometry. The composition of the vitamers were α-tocopherol (14-17%), α-tocotrienol (22-24%), γ- tocotrienol (49-53%), α- tocotrienol (6-7%) and α- tocomonoenol (3%) throughout the physical refining. The concentration of all vitamers in crude palm oil was 1273±18 ppm. The concentrations of all vitamers in degummed palm oil, bleached palm oil and deodorized palm oil were 1134±20 ppm, 1095±18 ppm and 1029±18 ppm, respectively. This method provides fast and valuable information with minimal analysis time and no sample pre-treatment.

American Journal of Applied Sciences
Volume 4 No. 6, 2007, 374-377

DOI: https://doi.org/10.3844/ajassp.2007.374.377

Submitted On: 9 January 2007 Published On: 30 June 2007

How to Cite: Puah, C. W., Choo, Y. M., Ma, A. N. & Chuah, C. H. (2007). The Effect of Physical Refining on Palm Vitamin E (Tocopherol, Tocotrienol and Tocomonoenol) . American Journal of Applied Sciences, 4(6), 374-377. https://doi.org/10.3844/ajassp.2007.374.377

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Keywords

  • HPLC
  • palm oil
  • physical refining
  • α−tocomonoenol