Research Article Open Access

Detection of DNA Hypomethylation Mediated Floral Induction in Longan and Spinach Using the HAT-RAPD Technique

S. Anuntalabhochai, R. Chundet, N. Buapong and R. W. Cutler

Abstract

In this study, the HAT-RAPD technique was used to detect DNA methylation in the four plant species, rice (Oryza sativa), petunia (Petunia hybrida), spinach (Spinacea oleracea L.) and longan (Dimocarpus longan Lour.), which were treated using 5-azacytidine, potassium chlorate (KClO3) and a low temperature induction. Rice and petunia were chosen to be induced because in these species it is known that 5-azacytidine is able to induce hypomethylation in their genomes leading to morphological changes, in particular floral induction, in the developing plants. Using the HAT-RAPD technique, DNA methylation was detected using the restriction enzymes HpaII and MspI in rice and petunia (as expected) and in spinach, but was found to be absent in longan, which suggests that floral induction in longan is undetectable in longan.

American Journal of Applied Sciences
Volume 6 No. 2, 2009, 361-367

DOI: https://doi.org/10.3844/ajassp.2009.361.367

Submitted On: 17 June 2008 Published On: 28 February 2009

How to Cite: Anuntalabhochai, S., Chundet, R., Buapong, N. & Cutler, R. W. (2009). Detection of DNA Hypomethylation Mediated Floral Induction in Longan and Spinach Using the HAT-RAPD Technique . American Journal of Applied Sciences, 6(2), 361-367. https://doi.org/10.3844/ajassp.2009.361.367

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Keywords

  • Longan
  • spinach
  • hypomethylation
  • vernalization
  • HAT-RAPD
  • temperature dependence