Research Article Open Access

Screening of Lactic Acid Bacteria for Antifungal Activity against Aspergillus oryzae

Belal Jamal Muhialdin1 and Zaiton Hassan1
  • 1 Department of Science and Technology, University Sains Islam Malaysia Bandar Baru Nilai, 71800 Nilai Negeri Sembilan, Malaysia


Problem statement: The growth of spoilage fungi have been a global concern because of the economy loses and the health hazard of the mycotoxins produced by the spoilage fungi. Approach: A total of 137 lactic acid bacteria isolated from Malaysian fruits and fermented foods were screened for antifungal activity using dual agar overlay method and well method against A. oryzae. Results: 23 isolates showed inhibition activity after 72 h incubation at 30°C. Supernatant of three isolates with strong antifungal activity was evaluated by well method and they inhibited the growth of the fungi at 30°C for 72 h. LAB supernatant reduced the mass growth of Aspergillus oryzae when incubated for 7 days at 30°C. The isolates were identified using API 50CH as Lactobacillus brevis G004, Lactobacillus fermentum Te007 and Pediococcus pentosaceus Te010. Conclusion: The three isolates studied inhibited the growth of the mycelia and conidia germination of the fungi which indicate the possibility of using LAB isolates as biopreservative.

American Journal of Applied Sciences
Volume 8 No. 5, 2011, 447-451


Submitted On: 6 February 2011 Published On: 17 May 2011

How to Cite: Muhialdin, B. J. & Hassan, Z. (2011). Screening of Lactic Acid Bacteria for Antifungal Activity against Aspergillus oryzae. American Journal of Applied Sciences, 8(5), 447-451.

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  • A. oryzae
  • antifungal activity
  • API 50CHL
  • Lactic Acid Bacteria (LAB)
  • global concern
  • antifungal activity
  • isolates inhibited
  • spoilage moulds