Research Article Open Access

Simultaneous Determination of Amlodipine and Atorvastatin in Caduet® Tablets Using HPLC

Maha Abdalla Alnowaiser1
  • 1 Department of Chemistry, Faculty of Science, Princess Nora Bint Abdul Rahman University, Riyadh, India

Abstract

A simple, selective, sensitive and precise, simultaneous high performance liquid chromatographic analysis of tablets containing amlodipine and atorvastatin was described. Good chromatographic separation was achieved using a Perfectsilr Target ODS-3 (4.6 cm × 250 mm, 5 µm) and a mobile phase consisting of acetonitrile-phosphate buffer pH 4.5 (55:45, v/v) at a flow rate 1ML min-1. The ultraviolet detector was set at wavelength 237 nm. Amlodipine and atorvastatin were measured at 1.071 and 3.765 min, respectively. The linear ranges for amlodipine and atorvastatin were 1-10 and 5-50 µg mL-1, respectively. The recoveries of amlodipine and atorvastatin in pharmaceutical preparation were all greater than 98.5% and their relative standard deviations were less than 2.0%. The limits of detection were 0.19 and 1.25 µg mL-1 for amlodipine and atorvastatin, respectively.

American Journal of Applied Sciences
Volume 10 No. 8, 2013, 849-852

DOI: https://doi.org/10.3844/ajassp.2013.849.852

Submitted On: 12 October 2012 Published On: 24 July 2013

How to Cite: Alnowaiser, M. A. (2013). Simultaneous Determination of Amlodipine and Atorvastatin in Caduet® Tablets Using HPLC. American Journal of Applied Sciences, 10(8), 849-852. https://doi.org/10.3844/ajassp.2013.849.852

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Keywords

  • HPLC
  • Cardiovascular
  • Hyperlipidemia