Research Article Open Access

Synthesis and Biological Evaluation of 6-Hydroxy-4-Methyl-5,7-(Bis-p-ChlorophenylAzo) Coumarin

Dalal M. Ibrahim1, Juliana Jumal1 and Farah Wahida Harun1
  • 1 Faculty of Science and Technology, University Sains Islam Malaysia (USIM), Bandar Baru Nilai, 71800 Nilai, Negeri Sembilan, Malaysia

Abstract

6-hydroxy-4-methyl-5,7-(bis-p-chlorophenylazo)coumarinhas been synthesized  and characterizedby CHN elemental analysis, FTIR, 1H-NMR-spectroscopy andmass-spectral data. Cytotoxic screening by MTT assay was carried out on the compoundagainst breast cancer cells. The overall results from preliminary screeningprogram revealed that the cell proliferation was highly inhibited by 6-hydroxy-4-methyl-5,7-(bis-p-chlorophenylazo)coumarin with the value of 2.81%, at concentration of 30 µg mL-1 comparedwith the untreated control cells and also possessed a good chelating activitywith IC50 value 1.87 µg mL-1. It issuggested that the cytotoxic activity is affected by hydroxyl and halogengroups as these groups have high electron affinity and high electronegativity.

American Journal of Applied Sciences
Volume 13 No. 1, 2016, 1-6

DOI: https://doi.org/10.3844/ajassp.2016.1.6

Submitted On: 9 February 2015 Published On: 21 December 2015

How to Cite: Ibrahim, D. M., Jumal, J. & Harun, F. W. (2016). Synthesis and Biological Evaluation of 6-Hydroxy-4-Methyl-5,7-(Bis-p-ChlorophenylAzo) Coumarin. American Journal of Applied Sciences, 13(1), 1-6. https://doi.org/10.3844/ajassp.2016.1.6

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Keywords

  • Bis-Coumarins
  • Cytotoxic Activity
  • Breast Cancer