Research Article Open Access

Enzymatic Kinetics of Fatty Acids Methyl Ester from Kapok Seed Oil (Ceiba Petandra) Using Lipozyme TL IM

Erin Ryantin Gunawan1, Dedy Suhendra1 and Baiq Rizkia Miftahatul Hasanah1
  • 1 Department of Chemistry, Faculty Mathematics and Science, University of Mataram, Indonesia

Abstract

Synthesis of Fatty Acids Methyl Ester (FAME) utilizing oil extracted from kapok seeds (Ceiba petandra) and catalyzed by lipozyme TL. 1M via enzymatic kinetics transesterification was succesfully conducted A mixture of different FAME, widely known as biodiesel is one of the sources of renewable energy. A kinetic analysis was investigated to identify the reaction model and the rate equation and to calculate kinetic parameters. The result obtained showed that the enzymatic kinetics followed a Michaelis-Menten model. The transesterification kinetic followed the Ping-pong Bi-Bi mechanism characterized by the υmax, Km(KO), Km(Met) values of 3.06×10-5 mmol/min.mg catalyst, 0.95×102 ppm, 1.30×102 ppm, respectively.

American Journal of Applied Sciences
Volume 14 No. 12, 2017, 1146-1151

DOI: https://doi.org/10.3844/ajassp.2017.1146.1151

Submitted On: 4 September 2017 Published On: 2 January 2018

How to Cite: Gunawan, E. R., Suhendra, D. & Miftahatul Hasanah, B. R. (2017). Enzymatic Kinetics of Fatty Acids Methyl Ester from Kapok Seed Oil (Ceiba Petandra) Using Lipozyme TL IM. American Journal of Applied Sciences, 14(12), 1146-1151. https://doi.org/10.3844/ajassp.2017.1146.1151

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Keywords

  • Enzymatic
  • Kinetics
  • Fatty Acids Methyl Ester
  • Kapok Seed Oil