Research Article Open Access

Assessment of the Toxic Effects of Chitosan-Coated Magnetite Nanoparticles on Drosophila melanogaster

Doris Vela1, Jonathan Rondal2, Sofía Cárdenas3, José Gutiérrez-Coronado4, Eliza Jara5, Alexis Debut6 and Fernanda Pilaquinga5
  • 1 Facultad de Ciencias Exactas y Naturales, Laboratorio de Genética Evolutiva, Pontificia Universidad Católica del Ecuador, Quito, Ecuador
  • 2 Departamento de Ciencias de la Vida y la Agricultura, Universidad de las Fuerzas Armadas ESPE, Sangolquí, Ecuador
  • 3 Pontificia Universidad Católica del EcuadorFacultad de Ciencias Exactas y Naturales, Laboratorio de Nanotecnología, Pontificia Universidad Católica del Ecuador, Quito, Ecuador
  • 4 Universidad Autónoma de Madrid, Madrid, Spain
  • 5 Facultad de Ciencias Exactas y Naturales, Laboratorio de Nanotecnología, Pontificia Universidad Católica del Ecuador, Quito, Ecuador
  • 6 Centro de Nanociencias y Nanotecnología, Universidad de las Fuerzas Armadas ESPE, Sangolquí, Ecuador

Abstract

Nanoparticles are a cause for concern because of their potential toxic effects on human health and the environment. The aim of this study was to assess the toxic effect of chitosan-coated magnetite nanoparticles (11.00±4.7 nm) on Drosophila melanogaster through the observation of hemolymph composition, DNA damage, larval survival and lifespan of flies. Chitosan-coated magnetite nanoparticles were synthesized by co-precipitation method. Drosophila larvaes and adults were exposed to 500 and 1000 ppm nanoparticles solution. After exposure, each type of larval hemocytes was recognized. Comet assay was performed to detect the DNA damage in the hemocytes. Also, the larval survival and lifespan of exposed flies were observed. Our results showed the toxic effect of the chitosan-coated magnetite nanoparticles through the increment of hemocytes, the emergence of lamellocytes, the presence of apoptotic hemocytes and the DNA damage detected by comet assay. In addition, nanoparticles produce decreasing of larval survival and shortening of the mean and maximum lifespan. The toxic effect the chitosan-coated magnetite nanoparticles is directly associated with 1000 ppm. No DNA damage was observed at 500 ppm.

American Journal of Applied Sciences
Volume 17 No. 1, 2020, 204-213

DOI: https://doi.org/10.3844/ajassp.2020.204.213

Submitted On: 16 July 2020 Published On: 25 September 2020

How to Cite: Vela, D., Rondal, J., Cárdenas, S., Gutiérrez-Coronado, J., Jara, E., Debut, A. & Pilaquinga, F. (2020). Assessment of the Toxic Effects of Chitosan-Coated Magnetite Nanoparticles on Drosophila melanogaster. American Journal of Applied Sciences, 17(1), 204-213. https://doi.org/10.3844/ajassp.2020.204.213

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Keywords

  • Comet Assay
  • Chitosan
  • Magnetite Nanoparticles
  • Hemolymph
  • Lifespan