Research Article Open Access

Reactive Nitrogen Intermediates Production by Macrophages of Mycobacterium bovis-Infected Goats and Supplemented with Dyhidroxyvitamin D3 in vivo

Aurora Martinez-Romero1, Jose Luis Ortega-Sanchez2, Jose Ramon Hernandez-Salgado2, Margarita Yolanda Mendoza-Ramos3, Rafael Rodriguez-Martinez3, Maribel Cervantes-Flores1, Joel Lopez-Perez4, Norma Urtiz-Estrada1, Miguel Arenas-Vargas5 and Raul Villegas-Viscaino3
  • 1 Juarez University of the State of Durango, Mexico
  • 2 Regional Unit University of Arid Zones, Mexico
  • 3 Autonomy Agrarian University Antonio Narro, Mexico
  • 4 Center of Studies Justo Sierra, Mexico
  • 5 Autonomy Metropolitan University, Mexico

Abstract

Problem statement: Tuberculosis (TB) remains one of the world's major health problems. To evaluate in vivo indices of cellular sensitization, antigen-induced Reactive Nitrogen Intermediates (RNI) responses by blood mononuclear cells from Mycobacterium bovis BCG-infected goats supplemented with 1, 25 dyhidroxyvitamin D3 [1, 25-(OH)2D3]. Approach: An experimental, longitudinal and comparative study was planned. Twelve animals of goat cattle 20-to 24-month-old sannen selected themselves. Five samplings were made, previous to the inoculation (zero d), 3, 7, 14 and 21 d after applying the treatments. The mononuclear cells by the Ficoll-hypaque method were obtained. The RNI, nitrites and nitrates (NO2- and NO3-) were quantified by Enzyme-Linked Immunosorbent Assay (ELISA). Results: The treatment with the 1, 25(OH)2D3 stimulated the NO3- synthesis indicating, that by itself it is a good modulador of the micobacterial replication and in the treatment with M. bovis-BCG vaccine increased as a result to the treatment with 1,25(OH)2D3. The exhibition to M. bovis-BCG vaccine with the treatment with 1, 25(OH)2D3 was able to increase answer NO3- in exposed animals. Conclusion: The 1, 25(OH)2D3 stimulated in vivo the production of RNI in goats exposed to M. bovis BCG vaccine.

American Journal of Animal and Veterinary Sciences
Volume 4 No. 1, 2009, 6-13

DOI: https://doi.org/10.3844/ajavsp.2009.6.13

Submitted On: 28 October 2008 Published On: 31 March 2009

How to Cite: Martinez-Romero, A., Ortega-Sanchez, J. L., Hernandez-Salgado, J. R., Mendoza-Ramos, M. Y., Rodriguez-Martinez, R., Cervantes-Flores, M., Lopez-Perez, J., Urtiz-Estrada, N., Arenas-Vargas, M. & Villegas-Viscaino, R. (2009). Reactive Nitrogen Intermediates Production by Macrophages of Mycobacterium bovis-Infected Goats and Supplemented with Dyhidroxyvitamin D3 in vivo. American Journal of Animal and Veterinary Sciences, 4(1), 6-13. https://doi.org/10.3844/ajavsp.2009.6.13

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Keywords

  • M. bovis-BCG vaccine
  • 1,25 dihydroxyvitamin D3
  • nitrites
  • nitrates
  • goats