Research Article Open Access

Adipose-Derived Canine Mesenchymal Stem Cells in PLAY® Media

Arjun Kulkarni1, Bhavana N. V.2, Teena Mary Thomas2, Archana S.2, Mercy Jennis Pramod2, Ramesh Jangra3, Jyothsna A. Rao2 and Gururaj Rao2
  • 1 Mallya Aditi International School, Bangalore, Karnataka, India
  • 2 Department of Biotechnology, iCREST, International Stem Cell Service Limited, India
  • 3 Cessna Lifeline Veterinary Hospital, Bangalore, Karnataka, India

Abstract

In veterinary medicine, stem cell-based therapies present promise for addressing orthopedic and degenerative conditions in canines. Mesenchymal Stem Cells (MSCs) possess unique regenerative, immunomodulatory, and differentiation capabilities. However, their efficacy relies on optimal in-vitro conditions. Prolonged expansion over passages leads to a gradual loss of stem cell traits, highlighting the importance of optimized culture conditions and media supplements. This study focuses on optimizing culture conditions for adipose-derived canine MSCs using play®, an in-house product containing growth factors and bioactive modulators. Our study provides insights into cellular properties, growth kinetics, and differentiation ability aiming to enhance adipose-derived canine MSCs' therapeutic potential in the field of veterinary medicine. Adipose-derived canine MSCs (AD-cMSCs) were isolated from adipose tissue from a canine source with veterinary professionals at Cessna Lifeline Veterinary Hospital, with prior pet owner consent. AD-cMSCs were cultured in 10% PLAY® or 10% Fetal Bovien Serum-supplemented media. Population Doubling Time (PDT) assessed growth rates. Trilineage differentiation potential was evaluated with specific media and morphology was examined under microscopy. Pluripotency gene expression was performed using a semi-quantitative polymerase chain reaction. AD-cMSCs cultured with 10% play® showed accelerated growth, enhanced differentiation potential, and elevated pluripotency marker expression compared to those in 10% FBS. These findings underscore the efficacy of play® as a promising culture supplement for optimizing AD-cMSC expansion and advancing their therapeutic potential in veterinary regenerative medicine

American Journal of Animal and Veterinary Sciences
Volume 19 No. 3, 2024, 240-246

DOI: https://doi.org/10.3844/ajavsp.2024.240.246

Submitted On: 14 September 2023 Published On: 13 July 2024

How to Cite: Kulkarni, A., V., B. N., Thomas, T. M., S., A., Pramod, M. J., Jangra, R., Rao, J. A. & Rao, G. (2024). Adipose-Derived Canine Mesenchymal Stem Cells in PLAY® Media. American Journal of Animal and Veterinary Sciences, 19(3), 240-246. https://doi.org/10.3844/ajavsp.2024.240.246

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Keywords

  • Adipose-Derived Canine MSCs’
  • Population Doubling Time
  • Regenerative Medicine
  • Stem Cells
  • Tri-Lineage Differentiation