Adipose-Derived Canine Mesenchymal Stem Cells in PLAY® Media
- 1 Mallya Aditi International School, Bangalore, Karnataka, India
- 2 Department of Biotechnology, iCREST, International Stem Cell Service Limited, India
- 3 Cessna Lifeline Veterinary Hospital, Bangalore, Karnataka, India
Abstract
In veterinary medicine, stem cell-based therapies present promise for addressing orthopedic and degenerative conditions in canines. Mesenchymal Stem Cells (MSCs) possess unique regenerative, immunomodulatory, and differentiation capabilities. However, their efficacy relies on optimal in-vitro conditions. Prolonged expansion over passages leads to a gradual loss of stem cell traits, highlighting the importance of optimized culture conditions and media supplements. This study focuses on optimizing culture conditions for adipose-derived canine MSCs using play®, an in-house product containing growth factors and bioactive modulators. Our study provides insights into cellular properties, growth kinetics, and differentiation ability aiming to enhance adipose-derived canine MSCs' therapeutic potential in the field of veterinary medicine. Adipose-derived canine MSCs (AD-cMSCs) were isolated from adipose tissue from a canine source with veterinary professionals at Cessna Lifeline Veterinary Hospital, with prior pet owner consent. AD-cMSCs were cultured in 10% PLAY® or 10% Fetal Bovien Serum-supplemented media. Population Doubling Time (PDT) assessed growth rates. Trilineage differentiation potential was evaluated with specific media and morphology was examined under microscopy. Pluripotency gene expression was performed using a semi-quantitative polymerase chain reaction. AD-cMSCs cultured with 10% play® showed accelerated growth, enhanced differentiation potential, and elevated pluripotency marker expression compared to those in 10% FBS. These findings underscore the efficacy of play® as a promising culture supplement for optimizing AD-cMSC expansion and advancing their therapeutic potential in veterinary regenerative medicine
DOI: https://doi.org/10.3844/ajavsp.2024.240.246
Copyright: © 2024 Arjun Kulkarni, Bhavana N. V., Teena Mary Thomas, Archana S., Mercy Jennis Pramod, Ramesh Jangra, Jyothsna A. Rao and Gururaj Rao. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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Keywords
- Adipose-Derived Canine MSCs’
- Population Doubling Time
- Regenerative Medicine
- Stem Cells
- Tri-Lineage Differentiation