Research Article Open Access

Characterization of Epoxidized and Non-Epoxidized Fatty Diethanolamides by High Performance Liquid Chromatography and Gas Chromatography

Choy Sin Lee1 and Song Cang Lee2
  • 1 International Medical University, Malaysia
  • 2 University of Malaya, Malaysia

Abstract

Problem statement: This study was carried out for the characterization of epoxidized and non-epoxidized fatty diethanolamides by High Performance Liquid Chromatography (HPLC) and Gas Chromatography (GC). Approach: The fatty diethanolamides were synthesized by reacting 40% (w/w) of Epoxidized Palm Olein (EPOo) with 60% (w/w) Refined Bleached Deodorized Plam Kernel Olein (RBDPKOo) with Diethanolamine (DEA) at 110°C for 5 h. Results: The identities of the peaks were established by secondary standards that were derived from pure methyl esters. HPLC separated major non-epoxidized diethanolamide compounds, namely C10:0, C12:0, C14:0, C16:O, C18:1 and C18:0 and the molecular weights of the compounds were dentified by Liquid Chromatography-Mass Spectrometry (LC-MS). Conclusion: GC provided a more complete separation for the non-epoxidized diethanolamides, epoxidized diethanolamides, glycerol, Diethanolamine (DEA) and some minor compounds from the self-condensation of DEA and diethanolamides. Gas Chromatography-Mass Spectrometry (GC-MS) elucidated the chemical structure of the epoxidized diethanolamides.

American Journal of Pharmacology and Toxicology
Volume 5 No. 3, 2010, 133-138

DOI: https://doi.org/10.3844/ajptsp.2010.133.138

Submitted On: 7 September 2010 Published On: 30 September 2010

How to Cite: Lee, C. S. & Lee, S. C. (2010). Characterization of Epoxidized and Non-Epoxidized Fatty Diethanolamides by High Performance Liquid Chromatography and Gas Chromatography. American Journal of Pharmacology and Toxicology, 5(3), 133-138. https://doi.org/10.3844/ajptsp.2010.133.138

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Keywords

  • Diethanolamides
  • gas chromatography
  • gas chromatography-mass spectrometry
  • high performance liquid chromatography
  • liquid chromatography-mass spectrometry