Research Article Open Access

Plant Growth Promoting Activity of Actinomycetes Isolated from Soybean Rhizosphere

Aris Tri Wahyudi1, Jepri Agung Priyanto1, Resti Afrista1, Deni Kurniati1, Rika Indri Astuti1 and Alina Akhdiya2
  • 1 Bogor Agricultural University, Indonesia
  • 2 Research and Development Institute, Indonesia

Abstract

Plant Growth Promoting Rhizobacteria (PGPR) is considered as the biological agent for improving plant growth. One Group of PGPR that have an important role in growth promoting of plant is Actinomycetes. The objective of this study was to isolate and screen Actinomycetes isolated from soybean rhizosphere as growth promoter of soybean in vitro. Fifty-three Actinomycetes isolates have successfully been isolated from soybean rhizosphere using two media, mainly Humic acid Vitamin Agar (HVA) and starch casein agar (SCA). Among 53 isolates, 18 (34%) isolates were able to produce IAA in range of 2.08 ppm to 16.70 ppm. Growth promotion test of soybean in vitro using Ragdoll method resulted 7 Actinomycetes isolates that significantly enhanced 3 plant growth parameters, including hypocotyl and radicular length as well as the number of lateral roots. Of those 7 isolates of Actinomycetes, 5 isolates were able to grow on nitrogen-free medium and solubilize phosphate. Those 5 isolates also were found as non-pathogenic, based on the negative reaction in hypersensitivity test. Based on 16S rRNA sequence analysis, 5 selected Actinomycetes isolates were highly homolog with Streptomyces genera in different taxa of species and strains (similarity ≥99%). Our finding reveals a potent application of 5 Actinomycetes isolates as plant growth promoter in soybean agriculture.

OnLine Journal of Biological Sciences
Volume 19 No. 1, 2019, 1-8

DOI: https://doi.org/10.3844/ojbsci.2019.1.8

Submitted On: 21 September 2018 Published On: 5 January 2019

How to Cite: Wahyudi, A. T., Priyanto, J. A., Afrista, R., Kurniati, D., Astuti, R. I. & Akhdiya, A. (2019). Plant Growth Promoting Activity of Actinomycetes Isolated from Soybean Rhizosphere. OnLine Journal of Biological Sciences, 19(1), 1-8. https://doi.org/10.3844/ojbsci.2019.1.8

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Keywords

  • Actinomycetes
  • Rhizosphere
  • Plant Growth Promoting
  • IAA
  • 16S rRNA