@article {10.3844/ajabssp.2012.88.96,
article_type = {journal},
title = {A Cyanobacterium Synechocystis sp. PCC 6803 Glutaredoxin Gene (slr1562) Protects Escherichia coli against Abiotic Stresses},
author = {Gaber, Ahmed and El-Assal, Salah El-Din},
volume = {7},
year = {2012},
month = {Mar},
pages = {88-96},
doi = {10.3844/ajabssp.2012.88.96},
url = {https://thescipub.com/abstract/ajabssp.2012.88.96},
abstract = {Problem statement: Glutaredoxins (GRXs) are ubiquitous small heat stable glutathione-dependent oxidoreductase enzymes that play a crucial role in plant development and response to oxidative stress. Approach: Cyanobacterium Synechocystis strain PCC 6803 contains two genes (slr1562 and ssr2061) encoding glutaredoxins. In the present investigation the slr1562 gene (grxC) was isolated and characterized. Results: The results revealed that the amino acid sequence deduced from GrxC protein share high identity with those of GRXs from other organisms and contain the consensus GRX family domain with a CPFC active site. Northern blotting analysis revealed that the expression of slr1562 gene could be induced by oxidative and salt stresses. Moreover, the protein GrxC was successfully overexpressed as a soluble fraction in Escherichia coli JM109. The over-expression of GrxC in Escherichia coli cells significantly increased resistance of cells to oxidative, drought and salt stresses. Conclusion/Recommendations: These results suggest that the slr1562 gene could play an important role in regulating abiotic tolerance against oxidative, drought and salt stresses in different organisms.},
journal = {American Journal of Agricultural and Biological Sciences},
publisher = {Science Publications}
}