TY  - JOUR
AU  - Youcef, N. 
AU  - Saidi, D. 
AU  - Mezemaze, F. 
AU  - El-Mecherfi, K. E. 
AU  - Kaddouri, H. 
AU  - Negaoui, H. 
AU  - Chekroun, A. 
AU  - Kheroua, O. 
PY  - 2009
TI  - Cross Reactivity between Dromedary Whey Proteins and IgG Anti Bovine α-Lactalbumin and Anti Bovine β-Lactoglobulin 
JF  - American Journal of Applied Sciences
VL  - 6
IS  - 8
DO  - 10.3844/ajassp.2009.1448.1452
UR  - https://thescipub.com/abstract/ajassp.2009.1448.1452
AB  - Problem statement: Our aim was to enhance the data on antigenic properties of dromedary whey proteins. Approach: The identification of the whey proteins was carried by SDS-page and Reversed phase high performance liquid chromatography (RP-HPLC). The cross-reactivity of dromedary whey proteins with IgG anti bovine β-lactoglobulin and anti bovine α-lactalbumin, obtained by immunisation of Balb/c mice, was carried out by ELISA. Results: The SDS-page showed the presence of band corresponding to α-lactalbumin and albumin serum; this was confirmed by the chromatogram obtained by RP-HPLC, where we detected a pick corresponding to α-lactalbumin. There was a cross reaction of dromedary whey proteins with IgG anti bovin α-lactalbumin but it was very weak with IgG anti boin β-lactoglobulin. Conclusion: We detected a cross reactivity between dromedary whey proteins and IgG anti bovin α-lactalbumin.