@article {10.3844/ajavsp.2011.84.87,
article_type = {journal},
title = {Genetic Polymorphism Exon 9-11 at the Leptin Gene Receptor in Breeder Hens of Mazandaran Native Fowls},
author = {Abbasi, Hossein Ali and Gharavysi, Shahabodin and Abdullahpour, Rohullah},
volume = {6},
number = {2},
year = {2011},
month = {Sep},
pages = {84-87},
doi = {10.3844/ajavsp.2011.84.87},
url = {https://thescipub.com/abstract/ajavsp.2011.84.87},
abstract = {Problem statement: Leptin a 16 kD protein synthesized by white adipose tissue and involved in regulation of feed intake, energy balance, fertility and immune function. In order to evaluate the leptin gene receptor polymorphism, we have used a Restriction Fragment Length Polymorphism (RFLP) method. Approach: Blood samples were collected from randomly chosen 100 native fowls breeding station of Mazandaran. The DNA extraction was based on salting out method and used amplified polymerase chain reaction technique. Exon and intron 9-11 of the fowl leptin gene receptor was amplified to produce a 382 bp fragment. The PCR products were electrophoresed on 1% agarose gel and stained by etidium bromide. Results: Then, they were digested of amplicons with Tsp509I and revealed two alleles A and B. Data were analysed using PopGene 32 package. In this population, AA, AB, BB genotype have been identified with the 69.14, 30.16, 0.7% frequencies. A and B alleles frequencies were 0.84, 0.16, respectively. x2 test didn’t signify Hardy-Weinberg equilibrium in this population (p},
journal = {American Journal of Animal and Veterinary Sciences},
publisher = {Science Publications}
}