TY  - JOUR
AU  - Ghasemi, Younes 
AU  - Ebrahiminezhad, Alireza 
AU  - Rasoul-Amini, Sara 
AU  - Zarrini, Gholamreza 
AU  - Ghoshoon, Mohammad Bagher 
AU  - Raee, Mohammad Javad 
AU  - Morowvat, Mohammad Hossein 
AU  - Kafilzadeh, Farshid 
AU  - Kazemi, Aboozar 
PY  - 2008
TI  - An Optimized Medium for Screening of L-Asparaginase production by Escherichia coli
JF  - American Journal of Biochemistry and Biotechnology
VL  - 4
IS  - 4
DO  - 10.3844/ajbbsp.2008.422.424
UR  - https://thescipub.com/abstract/ajbbsp.2008.422.424
AB  - Purified L-asparaginase II from Escherichia coli has been supplied and employed in the acute leukemia and other malignant neoplasms chemotherapy. L-asparaginase II gene (ansB) in E. coli is under regulation and certain conditions is needed for expression of this gene. In this investigation ,the various concentrations of modified M9 medium ingredients and various carbon source were tested to optimize the medium for expression and identification of L-asparaginase in E. coli. Finally a semi-quantitative plate assay for L-asparaginase producing Escherichia coli is reported.