@article {10.3844/ajbbsp.2009.142.146, article_type = {journal}, title = {Purification of Chymotrypsin-Trypsin Inhibitor from Winged Bean Seeds using Single Step Immunoaffinity Column}, author = {Roy, Sanhita and Dutta, Samir Kumar}, volume = {5}, number = {3}, year = {2009}, month = {Sep}, pages = {142-146}, doi = {10.3844/ajbbsp.2009.142.146}, url = {https://thescipub.com/abstract/ajbbsp.2009.142.146}, abstract = {Problem statement: Winged bean is a rich source of protease inhibitors. The seeds of winged bean contain several serine protease inhibitors of which winged bean chymotrypsin-trypsin inhibitor (WbCTI) is of significant importance due to its dual inhibition of both chymotrypsin and trypsin. The purification of WbCTI has always been difficult and a time taking process that involved large number of steps and various purification columns. Approach: This study was focused on the single step method of purification of WbCTI to homogeneity from winged bean seeds using immunoaffinity column chromatography. Results: WbCTI was purified to homogeneity using anti-WbCTI antibody column in a single step which gave a single band on SDS-PAGE analysis and with almost one and half times more yield of purified protein than obtained from previously mentioned purification methods. Conclusion: This method using immunoaffinity column is high yielding and time saving process of purification. }, journal = {American Journal of Biochemistry and Biotechnology}, publisher = {Science Publications} }