TY  - JOUR
AU  - Roy, Sanhita 
AU  - Dutta, Samir Kumar 
PY  - 2009
TI  - Purification of Chymotrypsin-Trypsin Inhibitor from Winged Bean Seeds using Single Step Immunoaffinity Column
JF  - American Journal of Biochemistry and Biotechnology
VL  - 5
IS  - 3
DO  - 10.3844/ajbbsp.2009.142.146
UR  - https://thescipub.com/abstract/ajbbsp.2009.142.146
AB  - Problem statement: Winged bean is a rich source of protease inhibitors. 
The seeds of winged bean contain several serine protease inhibitors of which winged 
bean chymotrypsin-trypsin inhibitor (WbCTI) is of significant importance due to 
its dual inhibition of both chymotrypsin and trypsin. The purification of WbCTI 
has always been difficult and a time taking process that involved large number 
of steps and various purification columns. Approach: This study 
was focused on the single step method of purification of WbCTI to homogeneity 
from winged bean seeds using immunoaffinity column chromatography. Results: 
WbCTI was purified to homogeneity using anti-WbCTI antibody column in 
a single step which gave a single band on SDS-PAGE analysis and with almost one 
and half times more yield of purified protein than obtained from previously mentioned 
purification methods. Conclusion: This method using immunoaffinity 
column is high yielding and time saving process of purification.