@article {10.3844/ajbbsp.2017.167.175,
article_type = {journal},
title = {Enhanced Fatty Acid Production in Escherichia coli by Over-Expression of NADPH Generating Enzymes},
author = {Zhang, Huaiyuan and Zhou, Yongshuang and Chen, Haiqin and Chen, Yong Q. and Song, Yuanda},
volume = {13},
number = {4},
year = {2017},
month = {Oct},
pages = {167-175},
doi = {10.3844/ajbbsp.2017.167.175},
url = {https://thescipub.com/abstract/ajbbsp.2017.167.175},
abstract = {NADPH is an essential factor for Fatty Acid (FA) biosynthesis. The effect of NADPH generating enzymes [NADP+-dependent malic enzyme (NADP-ME), NADP+-dependent isocitrate dehydrogenase (NADP-IDH), Glucose 6-Phosphate Dehydrogenase (GPD) and 6-Phosphogluconate Dehydrogenase (PGD)] on FA biosynthesis was investigated in an engineered Escherichia coli BL21 △fadE/pTE. Among NADPH-generating enzymes, GPD and ICD increased total FA production by 60 and 16% respectively. Especially medium-chain FA production was increased up to 4.2 and 2.5 folds respectively. In addition, over-expression of the endogenous Thioesterase (TE) reduced Cyclopropane Fatty Acid (CFA) production by 40% and over-expressing of GPD and ICD further decreased CFA production by 23 and 30%, respectively compared to the TE over-expressed strain. Over-expression of TE appeared to be a good strategy to produce high quality biodiesel with medium-chain FAs as major Component (62%) and with Minimal Amount of CFA (4%). This study reveals that NADPH-generating enzyme GPD is associated with FA biosynthesis in E. coli, but not all reducing power generating enzymes, are involved in FA biosynthesis in bacteria.},
journal = {American Journal of Biochemistry and Biotechnology},
publisher = {Science Publications}
}