@article {10.3844/ajbbsp.2023.30.35,
article_type = {journal},
title = {Hibiscus Flower Extract and Niacinamide Show Enhanced Whitening Effects in Mouse Melanoma Cells (B16F10)},
author = {Choi, Jae Eun and Jang, Tae Su and Park, Jung Min and Park, Eun Ji and Kim, Jae Kyung},
volume = {19},
number = {1},
year = {2023},
month = {Feb},
pages = {30-35},
doi = {10.3844/ajbbsp.2023.30.35},
url = {https://thescipub.com/abstract/ajbbsp.2023.30.35},
abstract = {Melanoma, a type of cancer that most often occurs on skin exposed to UV rays, is becoming increasingly common. Recent research has focused actively on using natural products. The goal of this study was to compare the melanin-inhibitory effects of Hibiscus (Hibiscus sabdariffa L.) flower Extract (HE) and Niacinamide (NA), a functional ingredient with whitening effects, on melanoma cells. The 3-(4,5-dimethylthiazol-2-yl) -2,5-diphenyltetrazolium bromide (MTT) assay was used to determine the effects of HE (1, 3.5, 7, 10, and 14 μg/mL) and NA (1, 5, 10, 20, and 25 μg/mL) on cell viability. A lactate dehydrogenase cytotoxicity assay was employed to confirm the cytotoxic effect of HE and NA on mouse melanoma cells. The real-time reverse-transcription polymerase chain reaction was performed to analyze Tyrosinase (TYR), Tyrosinase-Related Protein (TRP), and Microphthalmia-associated Transcription Factor (MITF) mRNA expression levels to determine whitening effects. Vitamin C (VC, 10 µg/mL) was selected as a control. The MTT assay showed that HE and NA were non-cytotoxic at },
journal = {American Journal of Biochemistry and Biotechnology},
publisher = {Science Publications}
}