@article {10.3844/ajbbsp.2026.22.01.006, article_type = {journal}, title = {Preparation and Properties Study of PCM@ECD Bilayer}, author = {Zhao, Yan and Zhang, Heng and Zhao, Yanfei and Younas, Madiha and Rong, Qingfeng and Shao, Qing and Zhu, Lanlan}, volume = {22}, number = {1}, year = {2026}, month = {May}, pages = {6-1}, doi = {10.3844/ajbbsp.2026.22.01.006}, url = {https://thescipub.com/abstract/ajbbsp.2026.22.01.006}, abstract = {This study reports the fabrication of a core-shell active bilayer membrane (PCM@ECD) via a combined tape-casting and layer-transfer methodology. The inner core layer comprised Pectin-Chitosan (PC) microcapsules, synthesized by complex coacervation, encapsulating Orange Peel Essential Oil (OPEOM) with an embedding efficiency of 83.08%. The outer shell layer consisted of Ethyl Cellulose (EC) doped with zinc-doped carbon dots (Zn-CDs, average size: 1.72 nm). The microencapsulation of OPEOM facilitated the sustained release of its active components, primarily limonene, and significantly enhanced its stability. The incorporated Zn-CDs conferred potent UV-shielding properties, attributed to π→π* and n→π* electronic transitions across the 200–400 nm wavelength range. Optimized at loadings of 15% (w/w) OPEOM and 0.01% (w/w) Zn-CDs, the composite bilayer film demonstrated superior functional performance: enhanced tensile strength (28.7 ± 1.2 MPa), a 38.2% reduction in water vapor permeability (WVP), exceptional UV-blocking efficacy (>95% at 350 nm), significant antibacterial activity (inhibition rates of 87.0% and 66.7% against Shewanella putrefaciens and Pseudomonas sp., respectively), and high antioxidant capacity (79.97% DPPH radical scavenging). This work pioneers a synergistic preservation strategy utilizing Zn-CDs and OPEO microcapsules, demonstrating significant potential for application in high-moisture food systems.}, journal = {American Journal of Biochemistry and Biotechnology}, publisher = {Science Publications} }