TY - JOUR AU - Villalba-Magdaleno, J.D. AU - Rojas, R. AU - Gómez, C. AU - Shibayama, M. AU - Carrero, Julio C. AU - D.G., PY - 2007 TI - Emetine produce Entamoeba histolytica Death by Inducing a Programmed Cell Death JF - American Journal of Infectious Diseases VL - 3 IS - 2 DO - 10.3844/ajidsp.2007.110.114 UR - https://thescipub.com/abstract/ajidsp.2007.110.114 AB - Amoebiasis is a major public health problem in tropical and subtropical countries. Although a number of antiamoebic agents are used for its treatment and the molecular targets of these drugs have been identified, the biochemical and physiological mechanisms that produce trophozoite death are little known. The present study dates the in vitro induction of programmed cell death (PCD) in E. histolytica by emetine. Morphologically, the emetine incubation reduces cytoplasmic volume, produce nuclear condensation and DNA fragmentation, maintaining the plasma membrane integrity; biochemically, the morphological changes are in concordance with the overproduction of reactive oxygen species inside trophozoites. These results provide the first insight related the cellular mechanisms of emetine action.