TY  - JOUR
AU  - Rodríguez, José W. 
AU  - Pagan, Nat O. 
AU  - Ocasio, Mar C. 
AU  - Ríos, Zilka 
AU  - Cubano, Luis A. 
AU  - Boukli, Nawal M. 
AU  - Otero, Miguel 
AU  - Hunter, Robert 
AU  - Nair, Madhavan P. 
AU  - Rios-Olivares, Eddy 
PY  - 2007
TI  - Induction of a Soluble Anti-HIV-1 factor (s) with IFN-γ, IL-10, and β-Chemokine Modulating Activity by an Influenza-Bacterial Polyantigenic Mixture
JF  - American Journal of Infectious Diseases
VL  - 3
IS  - 4
DO  - 10.3844/ajidsp.2007.267.275
UR  - https://thescipub.com/abstract/ajidsp.2007.267.275
AB  - Partial immune restoration may be obtained with highly active antiretroviral therapy (HAART), but specific anti-HIV-1 immune responses do not appear to improve substantially. We have demonstrated that a soluble factor(s) induced by a mixture of inactivated influenza and bacterial vaccines called polyantigenic immunomodulator (PAI), possesses strong immunoregulatory and anti- HIV-1 activities. In the present study, we show that culture fluids from both PAI-stimulated peripheral blood mononuclear cells (PBMC) and CD8+ T-cells of HIV-1 infected patients were able to suppress HIV-1 replication in an MHC-unrestricted fashion. The PAI-induced antiviral activity was eliminated when culture fluids were pre-heated at 100°C for 10 min. and it is associated with induction of IFN-γ, MIP-1α, MIP-1β, and RANTES production, but inhibition of IL-10. Furthermore, this induction is dependent on the immunological status (CD4:CD8 ratio) of the HIV-1 infected patient. Taken together, our results suggest that the MHC-unrestricted HIV-1 suppression that is induced by culture fluids from PAI-stimulated PBMC may result from the stimulation of immune cell subpopulations to produce a heat-labile antiviral soluble factor(s), which in turn modulate cytokine and β-chemokine production. The identification of this PAI-induced soluble factor(s) may have major therapeutic potential.