TY  - JOUR
AU  - Acharya, Hari Shanker 
AU  - Sharma, Vimal 
PY  - 2009
TI  - Molecular Characterization of Opium Poppy (Papaver somniferum) Germplasm
JF  - American Journal of Infectious Diseases
VL  - 5
IS  - 2
DO  - 10.3844/ajidsp.2009.148.153
UR  - https://thescipub.com/abstract/ajidsp.2009.148.153
AB  - Problem statement: The medicinal value of opium poppy (2n = 22, 
Papaver somniferum L.) is due to presence of more than two dozen alkaloids. The 
genetic origin and diversity of cultivars used in the breeding collection is not 
fully known. To evaluate the genetic diversity of the 24 germplasm currently cultivated 
in the state to provide genetic information about heterogeneity, using RAPD and 
ISSR markers. Approach: Isolated genomic DNA was subjected to 
PCR amplification using either random or ISSR markers. The amplified fragments 
were scored and matrix was prepared. Jaccard similarity coefficients were calculated 
and dendogram was generated. Results: RAPD with 12 primers gave 
46 scorable fragments out of which 32 bands were polymorphic (69.52%) with 2.6 
polymorphic band per primer, while in ISSR analysis 9 primer gave good amplification 
with DNA of 24 opium poppy germplasm. They gave 27 scorable polymorphic with 3.0 
bends per primer. Dendrogram constructed revealed that the based on RAPD the germplasm 
cultivated comprised of one major group A and one minor group B, while two clusters 
of two germplasm each stands apart, on the basis of ISSR they fall into one major 
group A and one minor groups B, while one germplasm clusters of UOP-60 stands 
apart. Based on combined RAPD and ISSR analysis these germplasm were divided into 
1 major group A and 3 minor group B, C, D whereas one genotype UOP-6 stood apart. 
Conclusion: These results showed that the majority of cultivated 
germplasm fall in one cluster and have low genetic diversity.