TY  - JOUR
AU  - Krishnaraju, Alluri V. 
AU  - Rao, Chirravuri V. 
AU  - Rao, Tayi V.N. 
AU  - Reddy, K.N. 
AU  - Trimurtulu, Golakoti 
PY  - 2009
TI  - In vitro and In vivo Antioxidant Activity of Aphanamixis polystachya Bark
JF  - American Journal of Infectious Diseases
VL  - 5
IS  - 2
DO  - 10.3844/ajidsp.2009.60.67
UR  - https://thescipub.com/abstract/ajidsp.2009.60.67
AB  - Problem statement: Free radical stress leads to tissue injury 
and progression of disease conditions such as arthritis, hemorrhagic shock, atherosclerosis, 
diabetes, hepatic injury, aging and ischemia, reperfusion injury of many tissues, 
gastritis, tumor promotion, neurodegenerative diseases and carcinogenesis. Safer 
antioxidants suitable for long term use are needed to prevent or stop the progression 
of free radical mediated disorders. Approach: Many plants possess 
antioxidant ingredients that provided efficacy by additive or synergistic activities. 
A. polystachya bark was a strong astringent, used for the treatment of liver and 
spleen diseases, rheumatism and tumors. Antioxidant activity of the crude extracts 
of bark of A. polystachya were assessed using NBT, DPPH, ABTS and FRAP assays. 
The potent fraction (AP-110/82C) was tested for in vivo efficacy Results: 
The methanol, aqueous methanol and water extracts exhibited potent antioxidant 
activity compared to known antioxidants. In vivo studies on potent fraction AP-110/82C 
demonstrated dose dependent reduction in hepatic malondialdehyde (320.6, 269.3 
and 373.69 μM mg-1 protein) with simultaneous improvement in hepatic glutathione (6.9, 17.1 and 5.8 μg mg-1 protein) and catalase levels (668.9, 777.0 and 511.94 μg mg-1 protein) respectively for 50, 100 mg kg-1 doses and control) compared to control group. Conclusion: Due to its natural origin and potent free-radical scavenging ability A. polystachya could be used as a potential preventive intervention for free radical-mediated diseases.