TY  - JOUR
AU  - Krishnaraju, Alluri V. 
AU  - Rao, Chundi B.M. 
AU  - Sundararaju, Dodda 
AU  - Sengupta, Krishanu 
AU  - Trimurtulu, Golakoti 
PY  - 2009
TI  - Anti-Inflammatory Activity of Vitex leucoxylon L. Bark Extracts Against Freund's Complete Adjuvant Induced Arthritis in Sprague Dawley Rat
JF  - American Journal of Infectious Diseases
VL  - 5
IS  - 2
DO  - 10.3844/ajidsp.2009.68.73
UR  - https://thescipub.com/abstract/ajidsp.2009.68.73
AB  - Problem statement: Anti-inflammatory agents, particularly steroids 
and cyclooxygenase inhibitors are often associated with adverse side effects including, 
GI irritation, ulcers, hypertension and cardiac abnormalities. A safe and effective 
intervention is essential for the treatment of inflammatory disorders. Approach: 
Abstract Vitex leucoxylon L., a medicinal plant of the verbenaceae family, 
used in traditional medicine for relieving headache and catarrh. VL-89/185A, was 
obtained by bio activity-guided fractionation using 5-Lipoxygenase inhibitory 
activity. VL-89/185A was further tested against Freund's Complete Adjuvant (FCA) 
induced arthritis in Sprague Dawley (SD) rat. Results: Oral supplementation 
of VL-89/185A resulted in significant anti-inflammatory effects as indicated by 
reduction in paw edema both at 100 and 250 mg kg-1 doses when compared to untreated 
control rats. Furthermore, treatment with VL-89/185A significantly reduced circulating 
proinflammatory cytokines TNF-α and IL-1β. The safety of VL-89/185A was 
established (LD50 >5000 mg kg-1 body) by acute oral toxicity limit test according 
to OECD guidelines 425. Conclusion: The safety and efficacy profiles 
indicated that VL-89/185A is a safe intervention for inflammatory disorders.