TY - JOUR AU - Ahmadi, Kazem AU - McCruden, Alan B. PY - 2005 TI - Androgen Receptor in Macrophages of Male Rat is Greater Than in Female JF - American Journal of Immunology VL - 1 IS - 1 DO - 10.3844/ajisp.2005.48.54 UR - https://thescipub.com/abstract/ajisp.2005.48.54 AB - The presence and possible sex differences of androgen receptor in peritoneal macrophages was investigated using immunomagnetic beads. Macrophages were incubated with different concentrations of [3H]-5αDHT in the presence or absence of a 100 fold excess of unlabelled 5α-DHT. Labelled cells were separated from unbound steroid by immunomagnetic beads coated with anti-rat macrophage antibody. The binding identified in the rat macrophages was highly selective towards androgenic compounds. The dissociation constant (kd) value for the receptor was calculated to be 3.3X10-9M and 5X10-9M for macrophages of male and female rat respectively. The number of receptors in each cell was 792±3 and 120±1 for male and female respectively. Indicating a sex differences in androgen receptor (p<0.001). Taken together it can be concluded that part of sex differences in immune responses and also auto-immune disease could be related to sex differences in androgen receptor in macrophages.