@article {10.3844/ajmsp.2015.31.39,
article_type = {journal},
title = {Optimization of Cultural Conditions for Production of Exopolysaccaride by Halomonas marina HMA 103 under Batch-Culture},
author = {Pal, Arundhati and Biswas, Amrita and Chatterjee, Sriya and Paul, A. K.},
volume = {6},
year = {2015},
month = {Jul},
pages = {31-39},
doi = {10.3844/ajmsp.2015.31.39},
url = {https://thescipub.com/abstract/ajmsp.2015.31.39},
abstract = {A moderately halophilic bacterium, Halomonas marina HMA 103 (MTCC 8968) accumulating intracellular polyesters also produced significant amount of exopolysaccharides (EPS) under batch-culture. Production of EPS by the bacterium was more or less parallel with the growth in modified basal synthetic medium. During growth, it produced 0.46 g/L of EPS after 50 h of incubation and was accompanied by the complete utilization of glucose from the medium. EPS production was maximum in 2% (w/v) glucose followed by sucrose and maltose and was positively influenced by yeast extract, beef extract and peptone. Optimum concentrations of sulfate and phosphate for EPS production were 2.4% (w/v) and 0.03% (w/v) respectively. High concentration (10% w/v) of NaCl stimulated both growth and EPS production by H. marina HMA 103. Characteristically, this EPS is a heteropolymer of glucose and mannose and the Fourier Transform Infrared (FTIR) spectrum showed distinct absorption peaks at 3424.2, 1638.7, 1122.9 and 622.3 cm-1 indicating the presence of free hydroxyl, phenyl or carbonyl groups of carbohydrate. Further, the detailed physico-chemical analysis will indicate the significance and potential importance of this EPS in biotechnology.},
journal = {Current Research in Microbiology},
publisher = {Science Publications}
}