Research Article Open Access

Bacteriological Examination of Computer Keyboards and Mouse Devices and Their Susceptibility Patterns to Disinfectants

Onochie Charles Chimezie1, Anyim Chukwudi2, Alo Moses Nnaemeka3, Onwa Ndubuisi Collins2, Okonkwo Eucharia Chinyere2 and Afiukwa Felicitas Ngozi2
  • 1 Nnamdi Azikiwe University Awka, Nigeria
  • 2 Ebonyi State University, Nigeria
  • 3 Federal University Ndufu Alike-Ikwo, Nigeria


Computers are ubiquitous and have been shown to be contaminated with potentially pathogenic bacteria in some communities. There is no economical way to test all the keyboards and mouse out there, but there are common-sense ways to prevent bacterial contamination or eliminate it if it exists. In this study, swabs specimens were collected from surfaces of 250 computer keyboards and mouse and plated on different bacteriological media. Organisms growing on the media were purified and identified using microbiological standards. It was found that all the tested computer keyboards and mouse devices, were positive for microbial contamination. The percentages of isolated bacteria (Staphylococcus spp., Escherichia spp., Pseudomonas spp. and Bacillus spp.) were 43.3, 40.9, 30.7, 34.1, 18.3, 18.2, 7.7 and 6.8% for computer keyboards and mouse respectively. The isolated bacteria were tested against the 6 different disinfectants (Dettol, Isol, Izal, JIK, Purit and Septol®). Antibacterial effects of the disinfectants were also concentration dependent. The agar well diffusion technique for determining Minimum Inhibitory Concentration (MIC) was employed. The Killing rate (K) and Decimal Reduction Time (DRT) of the disinfectants on the organism were also determined. The overall result of this study showed that Dettol®, followed by JIK® was highly effective against all the bacterial isolates tested while Septol and Izal® were least effective. Isol and Purit® showed moderate antibacterial effects. Keyboards and mouse should be disinfected daily. However, it is recommended that heightened surveillance of the microbial examination of computer keyboards should be undertaken at predetermined intervals.

Current Research in Microbiology
Volume 4 No. 1, 2013, 9-19


Submitted On: 26 January 2013 Published On: 19 January 2013

How to Cite: Chimezie, O. C., Chukwudi, A., Nnaemeka, A. M., Collins, O. N., Chinyere, O. E. & Ngozi, A. F. (2013). Bacteriological Examination of Computer Keyboards and Mouse Devices and Their Susceptibility Patterns to Disinfectants. Current Research in Microbiology, 4(1), 9-19.

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  • Bacteria
  • Computer
  • Disinfectants
  • Antibacterial Activity
  • MIC
  • DRT
  • Killing Rate