Research Article Open Access

Optimization of Cultural Conditions for Production of Exopolysaccaride by Halomonas marina HMA 103 under Batch-Culture

Arundhati Pal1, Amrita Biswas2, Sriya Chatterjee2 and A. K. Paul2
  • 1 Serampore College, India
  • 2 University of Calcutta, India

Abstract

A moderately halophilic bacterium, Halomonas marina HMA 103 (MTCC 8968) accumulating intracellular polyesters also produced significant amount of exopolysaccharides (EPS) under batch-culture. Production of EPS by the bacterium was more or less parallel with the growth in modified basal synthetic medium. During growth, it produced 0.46 g/L of EPS after 50 h of incubation and was accompanied by the complete utilization of glucose from the medium. EPS production was maximum in 2% (w/v) glucose followed by sucrose and maltose and was positively influenced by yeast extract, beef extract and peptone. Optimum concentrations of sulfate and phosphate for EPS production were 2.4% (w/v) and 0.03% (w/v) respectively. High concentration (10% w/v) of NaCl stimulated both growth and EPS production by H. marina HMA 103. Characteristically, this EPS is a heteropolymer of glucose and mannose and the Fourier Transform Infrared (FTIR) spectrum showed distinct absorption peaks at 3424.2, 1638.7, 1122.9 and 622.3 cm-1 indicating the presence of free hydroxyl, phenyl or carbonyl groups of carbohydrate. Further, the detailed physico-chemical analysis will indicate the significance and potential importance of this EPS in biotechnology.

Current Research in Microbiology
Volume 6 No. 2, 2015, 31-39

DOI: https://doi.org/10.3844/ajmsp.2015.31.39

Submitted On: 26 February 2015 Published On: 27 July 2015

How to Cite: Pal, A., Biswas, A., Chatterjee, S. & Paul, A. K. (2015). Optimization of Cultural Conditions for Production of Exopolysaccaride by Halomonas marina HMA 103 under Batch-Culture. Current Research in Microbiology, 6(2), 31-39. https://doi.org/10.3844/ajmsp.2015.31.39

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Keywords

  • Halomonas
  • Moderately halophilic bacteria
  • Exopolysaccharides
  • Solar saltern
  • Batch-culture