Research Article Open Access

Androgen Receptor in Macrophages of Male Rat is Greater Than in Female

Kazem Ahmadi1 and Alan B. McCruden2
  • 1 Baqiyatallah Medical Science University, United Kingdom
  • 2 University of Strathclyde, United Kingdom

Abstract

The presence and possible sex differences of androgen receptor in peritoneal macrophages was investigated using immunomagnetic beads. Macrophages were incubated with different concentrations of [3H]-5αDHT in the presence or absence of a 100 fold excess of unlabelled 5α-DHT. Labelled cells were separated from unbound steroid by immunomagnetic beads coated with anti-rat macrophage antibody. The binding identified in the rat macrophages was highly selective towards androgenic compounds. The dissociation constant (kd) value for the receptor was calculated to be 3.3X10-9M and 5X10-9M for macrophages of male and female rat respectively. The number of receptors in each cell was 792±3 and 120±1 for male and female respectively. Indicating a sex differences in androgen receptor (p<0.001). Taken together it can be concluded that part of sex differences in immune responses and also auto-immune disease could be related to sex differences in androgen receptor in macrophages.

American Journal of Immunology
Volume 1 No. 1, 2005, 48-54

DOI: https://doi.org/10.3844/ajisp.2005.48.54

Submitted On: 20 February 2006 Published On: 31 March 2005

How to Cite: Ahmadi, K. & McCruden, A. B. (2005). Androgen Receptor in Macrophages of Male Rat is Greater Than in Female. American Journal of Immunology, 1(1), 48-54. https://doi.org/10.3844/ajisp.2005.48.54

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Keywords

  • Macrophage androgen receptor
  • immunomagnetic
  • male and female