Research Article Open Access

Bio-Safety Technology in Production of Bovine Herpesvirus Type 5 (BoHV-5) Using an Alternative Serum-Free Medium

Tereza C. Cardoso1, Heitor F. Ferrari1, Maria Cecília R. Luvizotto1 and Clarice W. Arns1
  • 1 Universidade Estadual Paulista, Brazil

Abstract

The detection and growth of bovine herpes virus type 5 (BoHV-5) was evaluated in three different media without foetal bovine serum or animal protein by infecting three different cell lines. The OPTI-PRO, VP-SFM and RPMI 1640 media were supplemented by L-glutamine, antibiotics added by 5µg of insulin-like growth factor (IGF-I) and used to adapt CER, MDBK and CRIB cells in a statistic continuous culture system. The results obtained by CRIB and MDBK cells adaptation showed steady growth after 10th passages in OPTI-PRO medium, 20th passages in VP-SFM medium and 30th passages in RPMI 1640 medium, respectively. The OPTI-PRO and RPMI 1640 media were not able to support CER growth, even supplemented by IGF-1 and present apoptotic cells at 72h post-infection. The CER cells seeded after adaptation by 10th passages in VP-SFM medium added by 5µg/20ml of IGF-1 growth factor revealed the best virus titres compared to MDBK and CRIB cell lines. It was concluded that CER, MDBK and CRIB cells infected by BoHV-5 serotype could be used for laboratory diagnosis propagated on much safer culture system of high biotechnology advances.

American Journal of Biochemistry and Biotechnology
Volume 3 No. 3, 2007, 125-130

DOI: https://doi.org/10.3844/ajbbsp.2007.125.130

Submitted On: 21 April 2007 Published On: 30 September 2007

How to Cite: Cardoso, T. C., Ferrari, H. F., Luvizotto, M. C. R. & Arns, C. W. (2007). Bio-Safety Technology in Production of Bovine Herpesvirus Type 5 (BoHV-5) Using an Alternative Serum-Free Medium. American Journal of Biochemistry and Biotechnology, 3(3), 125-130. https://doi.org/10.3844/ajbbsp.2007.125.130

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Keywords

  • Bovine Herpesvirus
  • BoHV-5
  • CER cells
  • MDBK cells
  • CRIB cells
  • serum-free media