Research Article Open Access

Plasmid-mediated Detoxification of Mycotoxin Zearalenone in Pseudomonas Sp. ZEA-1

Abdullah D. Altalhi1
  • 1 Taif University, Saudi Arabia

Abstract

The Pseudomonas sp. Strain ZEA-1 was isolated from rhizosphere of corn plant by an enrichment technique showed capability of utilizing zearalenone as the sole source of carbon. The bacterium rapidly utilized zearalenone beyond 200 µg/ml and showed prolific growth in a minimal medium containing 100 µg/ml zearalenone source. The course of ZEA degradation as well as the formation of its metabolites was observed by UV Spectrophotometer and thin layer chromatography analysis. Toxicity of biotransformation products of zearalenone was tested in a toxicity assay with the shrimp Artemia salina. Residual toxicity of these metabolites against A.salinia was reduced. The wild type strain ZEA-1 transferred zearalenone degradation property to E.coli strain. The transconjugant harboured a plasmid of the same molecular size (approximately 120 Kb) as that of donor strain, while cured strain was plasmidless and did not utilize zearalenone.

American Journal of Biochemistry and Biotechnology
Volume 3 No. 3, 2007, 150-158

DOI: https://doi.org/10.3844/ajbbsp.2007.150.158

Submitted On: 7 February 2007 Published On: 30 September 2007

How to Cite: Altalhi, A. D. (2007). Plasmid-mediated Detoxification of Mycotoxin Zearalenone in Pseudomonas Sp. ZEA-1. American Journal of Biochemistry and Biotechnology, 3(3), 150-158. https://doi.org/10.3844/ajbbsp.2007.150.158

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Keywords

  • Zearalenone
  • biotransformation
  • E. coli
  • Pseudomonas sp. Artemia salin