Research Article Open Access

Immobilization of Lipase from Candida Rugosa on Palm-Based Polyurethane Foam as a Support Material

Roila Awang1, Mohd Rafaei Ghazuli2 and Mahiran Basri2
  • 1 Persiaran Institusi, Malaysia
  • 2 Universiti Putra Malaysia, Malaysia

Abstract

Lipase from Candida rugosa was immobilized onto palm-based polyurethane foam (PU), which the polymer was pre-soaked in co-immobilized agent. The activities of PU-immobilized lipase were tested by the esterification reaction of oleic acid and oleyl alcohol in hexane. The PU-immobilized lipase was then characterized in term of its thermal, operational and storage stability. The optimum temperature for native and PU-immobilized lipase was at 40°C. This shows that the immobilization did not alter the general character of the lipase. The PU-immobilized lipase shows different enzymatic characteristic-incubation time, enzyme concentration, solvent and operational stability compared to Lipozyme IM. The reuse stability of PU-immobilized lipase was at least four cycles. The % conversion above 80% was still achieved for the sample stored at -5°C after 9 days storage.

American Journal of Biochemistry and Biotechnology
Volume 3 No. 3, 2007, 163-166

DOI: https://doi.org/10.3844/ajbbsp.2007.163.166

Submitted On: 18 April 2007 Published On: 30 September 2007

How to Cite: Awang, R., Ghazuli, M. R. & Basri, M. (2007). Immobilization of Lipase from Candida Rugosa on Palm-Based Polyurethane Foam as a Support Material. American Journal of Biochemistry and Biotechnology, 3(3), 163-166. https://doi.org/10.3844/ajbbsp.2007.163.166

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Keywords

  • Candida rugosa
  • enzyme
  • immobilization
  • polyurethane