Research Article Open Access

Carica papaya Fruit Attenuates Apoptotic Degeneration After Permanent Cerebral Ischemia Rat

Pratchaya Kaewkaen1
  • 1 Burapha University, Thailand

Abstract

Apoptotic neurodegeneration is a phenomenon following cerebral ischemia. Both the increased caspase-3 and decreased Bcl-2 expression are reported to play crucial role on apoptosis in neuron. In addition, phytonutrient can successfully increase the endogenous antioxidant and lower the apoptotic responses. Therefore, anti-apoptotic effect of Carica papaya fruits was examined. To the best of our knowledge, no scientific effect was available. Therefore this study aimed to determine the effect of Carica papaya on neuron density and the densities of both caspase-3 and Bcl-2 immunopositive neuron in hippocampus. Adult male Wistar rats, weighing 280-320 g, were orally given Carica papaya fruits extract at doses of 50, 150 and 450 mg/kg at a period of 14 days before and 14 days after the occlusion of right Middle Cerebral Artery (Rt.MCAO). The results showed that Carica papaya fruits at high doses used in this study significantly attenuated the decreased neuron density in hippocampus. In addition, the high doses of Carica papaya could decrease caspase-3 immunopositive neuron density but increased density of Bcl-2 immunopositive neurons in hippocampus. The decreased caspase-3 immunopositive neuron density and the increased Bcl-2- immunopositive neurons might be partly responsible for neuroprotective effect of Carica papaya. Therefore, Carica papaya is the potential fruit to protect against apoptotic neurodegeneration following cerebral ischemia. However, further researches are essential to elucidate the possible mechanism.

American Journal of Biochemistry and Biotechnology
Volume 14 No. 4, 2018, 255-261

DOI: https://doi.org/10.3844/ajbbsp.2018.255.261

Submitted On: 21 July 2018 Published On: 22 November 2018

How to Cite: Kaewkaen, P. (2018). Carica papaya Fruit Attenuates Apoptotic Degeneration After Permanent Cerebral Ischemia Rat. American Journal of Biochemistry and Biotechnology, 14(4), 255-261. https://doi.org/10.3844/ajbbsp.2018.255.261

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Keywords

  • Carica papaya
  • Apoptosis
  • Neurodegeneration
  • Cerebral Ischemia