Research Article Open Access

Novel D-Galactose Isomerases from Lactobacillus Strains Isolated from the Sweet Sap of Agave atrovirens

María Miriam Hernández-Arroyo1, Miguel Ángel Plascencia-Espinosa2, María Eugenia Hidalgo-Lara3, Mariana Tinajero-Trejo4, Emilio Méndez-Merino5 and Sergio Rubén Trejo-Estrada6
  • 1 Centro de Investigación en Biotecnología Aplicada del Instituto Politécnico Nacional, Mexico
  • 2 Centro de Investigaci�n en Biotecnolog�a Aplicada del Instituto Polit�cnico Nacional, Mexico
  • 3 Av. Instituto Politécnico Nacional, Mexico
  • 4 Cell Biology Program, Canada
  • 5 Sigma Alimentos, Mexico
  • 6 Centro de Investigación en Biotecnología Aplicada del Instituto Politécnico Nac, Mexico

Abstract

D-tagatose is a natural monosaccharide used as a low-calorie sugar substitute in food, beverage and pharmaceutical products. Although it is a rare sugar, it can manufacture by enzymatic isomerization of D-galactose Isomerase (D-GI). In this study, a screening was carried out to search microorganism producing D-GI in aguamiel. A rich selective medium was used in arabinose extracted from gum arabic. From 98 isolates obtained aguamiel of agave pulquero (Agave atrovirens), it was obtained 4 strains of Lactobacillus that producing D-GI and 2 strains of Bacillus sp. The Podi-20 strain was identified as Lactobacillus diolivorans based on 16S rRNA analysis, biological and biochemical characteristics. Furthermore, the gene encoding D-GI from L. diolivorans Podi-20 strain. Analysis of sequence revealed that the Open Reading Frame (ORF) of the araA gene consist of 1,428 pb that encodes a protein of 476 amino acid residues. The bioconversion yield of D-galactose to D-tagatose by the purified D-GI after 14 h at 60°C was 31.4%. D-tagatose can manufacture by enzymatic isomerization. This study contributes to new knowledge on D-GI from Lactobacillus strains, in particular those isolated from artisanal functional foods from Agave.

American Journal of Biochemistry and Biotechnology
Volume 14 No. 4, 2018, 272-284

DOI: https://doi.org/10.3844/ajbbsp.2018.272.284

Submitted On: 26 August 2018 Published On: 11 December 2018

How to Cite: Hernández-Arroyo, M. M., Plascencia-Espinosa, M. &., Hidalgo-Lara, M. E., Tinajero-Trejo, M., Méndez-Merino, E. & Trejo-Estrada, S. R. (2018). Novel D-Galactose Isomerases from Lactobacillus Strains Isolated from the Sweet Sap of Agave atrovirens. American Journal of Biochemistry and Biotechnology, 14(4), 272-284. https://doi.org/10.3844/ajbbsp.2018.272.284

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Keywords

  • L-Arabinose Isomerase
  • D-Tagatose
  • Lactobacillus
  • Aguamiel
  • Lactobacillus diolivorans